Ficin is a proteolytic enzyme widely used in biomedical and industrial applications due to its ability to break down proteins. It is commonly employed in tissue dissociation protocols, particularly in cell isolation procedures for research and therapeutic purposes. Ficin is also used in immunology for modifying cell surface proteins, such as in lymphocyte separation and rosette formation studies. In the food industry, it serves as a meat tenderizer by degrading collagen and other tough protein structures. Additionally, ficin has been explored for its wound debriding properties in medical treatments, helping to remove dead tissue and promote healing. Its broad substrate specificity makes it valuable in enzymatic digestion processes across various fields.