Ser-Tyr β-naphthylamide is primarily used in biochemical research as a substrate for studying enzyme activity, particularly for proteases and peptidases. It helps in detecting and measuring the enzymatic hydrolysis of peptide bonds, making it valuable in understanding enzyme kinetics and mechanisms. This compound is also utilized in diagnostic assays to identify and characterize specific enzymes in biological samples. Its application extends to screening inhibitors of proteolytic enzymes, aiding in drug discovery and development. Additionally, it serves as a tool in studying protein metabolism and degradation pathways in various organisms.