This chemical is primarily used in biochemical research as a fluorogenic substrate for protease enzymes. It is particularly valuable in studying enzyme kinetics and activity, as the release of the 7-amido-4-methylcoumarin group upon cleavage by proteases produces a measurable fluorescent signal. This allows researchers to monitor protease activity in real-time, making it useful in assays to screen for protease inhibitors or to characterize enzyme specificity. Additionally, it can be applied in cell-based studies to investigate protease function in biological processes, such as apoptosis or signal transduction. Its stability and sensitivity make it a preferred choice for high-throughput screening and diagnostic applications in proteomics and drug discovery.