This chemical is primarily used in biochemical research as a chromogenic substrate for studying caspase-6 activity, an enzyme involved in apoptosis (programmed cell death). Ac-VEID-pNA TFA is cleaved by caspase-6, releasing p-nitroaniline (pNA), which can be measured spectrophotometrically at 405 nm. This assay helps in understanding the mechanisms of cell death and is often utilized to quantify caspase-6 enzymatic activity. Researchers employ it to investigate the role of caspases in various diseases, including neurodegenerative disorders and cancer. Its application is critical in drug discovery and development, particularly in identifying potential inhibitors or activators of caspase-6 for therapeutic purposes.