Used primarily in biochemical research, this compound serves as a substrate for studying enzyme activity, particularly in the context of proteases and peptidases. Its structure allows it to mimic natural peptide bonds, making it valuable for investigating enzyme kinetics and mechanisms. Additionally, it is employed in the development of assays to measure enzyme inhibition, aiding in drug discovery and the design of therapeutic agents targeting specific enzymes. Its nitro group also facilitates detection and quantification in experimental setups, enhancing its utility in analytical biochemistry.