GP-AMC is widely used as a fluorogenic substrate in biochemical assays to measure enzyme activity, particularly for proteases and peptidases. When the enzyme cleaves the substrate, it releases the fluorescent AMC (7-amino-4-methylcoumarin), which can be detected using fluorescence spectroscopy. This makes it a valuable tool in drug discovery and development, as it allows researchers to screen for enzyme inhibitors or activators. It is also employed in studying enzyme kinetics, understanding protease functions, and investigating cellular processes. Additionally, GP-AMC is used in diagnostic applications to detect enzyme-related diseases or abnormalities. Its sensitivity and specificity make it a preferred choice for high-throughput screening and research in molecular biology.