Used primarily in biochemical research, this compound serves as a substrate for enzyme activity assays, particularly in studies involving proteolytic enzymes like chymotrypsin. It helps in understanding enzyme kinetics and specificity by measuring the release of nitroaniline, which can be detected spectrophotometrically. Additionally, it is employed in the development of diagnostic tools for enzyme-related disorders, aiding in the identification and characterization of enzyme deficiencies or abnormalities. Its role in these applications contributes to advancements in both basic science and clinical diagnostics.