Gly-Gly hydrochloride is widely used in biochemical research as a dipeptide model to study peptide bond formation and hydrolysis. It serves as a substrate for enzymatic studies, particularly for proteases and peptidases, helping to understand their mechanisms and specificity. In pharmaceutical development, it is utilized as a building block for synthesizing more complex peptides and drug candidates. Additionally, it finds application in cell culture media as a nutrient source for microorganisms and mammalian cells, supporting their growth and metabolism. Its role in studying amino acid transport systems in cells is also significant, providing insights into cellular uptake mechanisms.