This chemical is primarily used in biochemical research as a fluorogenic substrate for protease enzymes, particularly thrombin. It is designed to measure protease activity by releasing a fluorescent compound upon enzymatic cleavage, allowing researchers to monitor and quantify enzyme kinetics in real-time. The application is especially valuable in studying blood coagulation mechanisms, as thrombin plays a critical role in this process. Additionally, it is utilized in drug discovery and development to screen and evaluate potential inhibitors of thrombin or other proteases, aiding in the creation of therapeutic agents for conditions like thrombosis. Its sensitivity and specificity make it a useful tool in both academic and industrial laboratories focused on enzymology and biomedical research.