Used as a fluorogenic substrate in enzymatic assays, particularly for detecting protease activity. It is especially useful in studying enzymes like trypsin or other serine proteases that cleave at arginine residues. Upon enzymatic cleavage, the compound releases 7-amido-4-methylcoumarin, which emits blue fluorescence under UV light, allowing real-time monitoring of enzyme kinetics and inhibition. Commonly applied in high-throughput screening, drug discovery, and biochemical research to assess protease function in cell lysates or purified enzyme preparations. Its high sensitivity and signal-to-noise ratio make it ideal for assays requiring precise quantification of enzymatic activity.