Used in biochemical research as a substrate for studying enzyme activity, particularly for proteases such as elastase and other peptidases. It helps in monitoring enzyme kinetics and inhibition due to its cleavable peptide bonds. Commonly employed in colorimetric or fluorometric assays when conjugated with a detectable tag, enabling the analysis of proteolytic processes in controlled environments. Also utilized in the development and validation of analytical methods for drug discovery where peptide hydrolysis is a key step.