Used in biochemical research as a dipeptide model for studying enzyme activity, particularly in relation to tyrosine metabolism and phosphorylation processes. Commonly employed in assays involving protein kinases and phosphatases due to the presence of phenolic hydroxyl groups. Also utilized in the development of synthetic substrates for proteolytic enzymes and in the design of peptide-based inhibitors. Shows application in neurochemistry studies because of structural similarity to endogenous neuropeptides. Employed in mass spectrometry calibration and peptide fragmentation studies.