Used in biochemical research as a substrate for studying enzyme activity, particularly in assays involving peptidases and proteolytic enzymes. Its structure makes it suitable for investigating enzyme specificity and inhibition kinetics. Commonly employed in diagnostic applications to assess renal and metabolic function due to its role as a derivative of hippuric acid, which is involved in detoxification pathways. Also utilized in the development of analytical methods for monitoring enzyme-catalyzed reactions in clinical and pharmaceutical settings.