Widely used in biochemical and enzymatic assays, this compound serves as a fluorogenic substrate for detecting protease activity, particularly for enzymes like trypsin and related serine proteases. Upon enzymatic cleavage, the molecule releases a highly fluorescent 7-amido-4-methylcoumarin (AMC) derivative, enabling real-time monitoring of enzyme kinetics with high sensitivity due to low background fluorescence. Its trifluoroacetate salt form enhances solubility and stability in aqueous assay buffers, making it suitable for high-throughput screening in drug discovery and diagnostic applications. It is also employed in studies of protein degradation, cell signaling, and apoptosis where precise measurement of proteolytic activity is required.