Used as a key substrate in enzymatic assays involving penicillin-binding proteins and bacterial cell wall synthesis studies. Plays a role in research on antibiotic resistance mechanisms, particularly in understanding how beta-lactam antibiotics interact with peptidoglycan precursors. Commonly employed in assays with DD-peptidases and transpeptidases to monitor enzyme activity and inhibition kinetics. Also utilized in the development and screening of novel antimicrobial agents targeting cell wall biosynthesis. Its structure mimics the natural terminal dipeptide of peptidoglycan, making it valuable in biochemical studies of bacterial cell division and wall assembly.