Suc-Leu-Tyr-AMC (Suc-LY-AMC) is a fluorogenic substrate widely used in biochemical research for studying protease activity. It is particularly suitable for measuring the activity of chymotrypsin-like serine proteases and cysteine proteases such as cathepsin L. Upon enzymatic cleavage at the Leu-Tyr bond, the AMC (7-amino-4-methylcoumarin) group is released, producing a quantifiable fluorescent signal. This feature makes it invaluable for enzyme kinetics studies, high-throughput screening in drug discovery, and investigating protease-related biological pathways. Its applications extend to cell-based assays and diagnostic research for monitoring protease activity in various disease models.