Gly-β-MCA is widely used in biochemical research as a fluorogenic substrate for detecting and measuring the activity of enzymes, particularly proteases like cathepsins and caspases. Its application is critical in studying enzyme kinetics, inhibitor screening, and understanding cellular processes involving proteolytic activity. In cell-based assays, it helps monitor enzyme activity in real-time, providing insights into cellular functions and disease mechanisms. Additionally, it is employed in drug discovery to evaluate the efficacy of protease inhibitors, making it a valuable tool in pharmaceutical research and development.