This chemical is primarily used in biochemical research as a fluorogenic substrate for studying protease activity. It is particularly valuable in assays designed to measure the enzymatic activity of caspases, which are crucial in apoptosis (programmed cell death). Researchers utilize it to monitor the cleavage of the peptide bond by caspases, as the release of the AMC (7-amino-4-methylcoumarin) group results in a measurable fluorescent signal. This application is essential for understanding cellular mechanisms involved in diseases like cancer, where apoptosis regulation is often disrupted. Additionally, it aids in the development and screening of potential therapeutic compounds targeting caspase pathways.