This peptide sequence is primarily used in biochemical research, particularly in the study of enzyme kinetics and protease activity. It serves as a substrate for various proteolytic enzymes, helping to elucidate their mechanisms and specificity. The inclusion of the EDDnp group makes it a quenched fluorescent substrate, allowing for the detection of enzymatic cleavage through fluorescence measurements. This application is valuable in drug discovery and development, where understanding enzyme behavior is crucial. Additionally, it can be utilized in the design of inhibitors or therapeutic agents targeting specific proteases involved in diseases.