L-Tyrosine 4-Nitroanilide is primarily used as a substrate in enzymatic assays, particularly for studying the activity of proteases and peptidases. It helps in measuring enzyme kinetics and identifying enzyme inhibitors, making it valuable in biochemical research and drug development. The compound’s nitroanilide group releases a colored product upon enzymatic cleavage, allowing for easy spectrophotometric detection. This property makes it a useful tool in high-throughput screening and diagnostic applications. Additionally, it aids in understanding enzyme mechanisms and specificity, contributing to advancements in enzymology and therapeutic target identification.