L-Arginine 7-Amido-4-methylcoumarin Dihydrochloride is primarily used in biochemical research as a fluorogenic substrate for the detection and study of enzyme activity, particularly proteases. Its application is significant in assays designed to measure protease activity, where the compound is cleaved by the enzyme, releasing a fluorescent product. This allows for the quantification and monitoring of enzymatic reactions in real-time, providing valuable insights into enzyme kinetics and inhibition. Additionally, it is employed in the development of high-throughput screening methods for identifying potential protease inhibitors, which are crucial in drug discovery and the study of various diseases. The compound’s fluorescent properties make it a useful tool in cellular and molecular biology research, aiding in the visualization and analysis of biological processes.