This peptide sequence is primarily used in biochemical research as a substrate for studying enzyme activity, particularly for proteases like thrombin. It is designed to release a fluorescent product upon cleavage, enabling researchers to monitor enzyme kinetics and activity in real-time. The compound is valuable in high-throughput screening assays to identify potential inhibitors or activators of specific enzymes. Its application extends to drug discovery, where it helps in the development of therapeutic agents targeting protease-related diseases. Additionally, it is utilized in diagnostic assays to measure enzyme levels in biological samples, aiding in the study of coagulation disorders and other medical conditions.