This peptide substrate is widely used in biochemical research, particularly in the study of proteolytic enzyme activity. It serves as a sensitive fluorogenic substrate for measuring the activity of proteases such as chymotrypsin and other serine or cysteine proteases. When cleaved by these enzymes, it releases a highly fluorescent AMC (7-amino-4-methylcoumarin) moiety, allowing for real-time monitoring of enzyme kinetics. Its application is crucial in drug discovery and development, where it aids in screening and characterizing potential protease inhibitors. Additionally, it is utilized in cellular and molecular biology studies to investigate protease functions in various biological processes, including apoptosis, signal transduction, and protein degradation pathways.