Used in fungal and yeast protein extraction protocols to prevent degradation of target proteins by endogenous proteolytic enzymes. The 100X concentrated mixture ensures rapid and broad-spectrum inhibition of proteases released during cell lysis, preserving protein integrity for downstream applications such as Western blotting, enzyme assays, and immunoprecipitation. Ideal for use in studies requiring high-quality, undegraded protein samples from tough-to-lyse fungal or yeast cells. Added directly to lysis buffers at 1X concentration for immediate protection upon cell disruption.