Used as an inhibitor of metalloproteases, particularly endothelin-converting enzyme (ECE), in biochemical and pharmacological research. It helps in studying the regulation of endothelin, a potent vasoconstrictor, making it valuable in cardiovascular research. Also employed to prevent the degradation of natriuretic peptides in experimental assays, improving the stability and detection of these peptides. Commonly added to enzyme assay buffers to inhibit unwanted proteolytic activity, especially when working with cell lysates or tissue extracts. Its selectivity makes it useful in distinguishing metalloprotease-mediated processes from other protease activities in cell signaling studies.