DAPT is widely used in biological research for its ability to modulate gamma-secretase activity, making it a key tool in studies related to cellular signaling pathways, particularly the Notch signaling pathway. It is commonly applied in stem cell research to influence cell differentiation and development, especially in neural and hematopoietic systems. DAPT is also utilized in cancer research due to its role in inhibiting Notch activation, which can affect tumor growth and progression in certain types of cancers. Additionally, it has been explored in studies related to Alzheimer’s disease, where gamma-secretase plays a role in the production of amyloid-beta peptides. Its reversible and selective inhibition allows for precise temporal control in experimental models, making it valuable in both in vitro and in vivo studies.