DAPT is widely used in biological research for its ability to modulate the Notch signaling pathway, which plays a critical role in cell differentiation, development, and apoptosis. It is commonly applied in stem cell studies to influence cell fate decisions, particularly in promoting neuronal differentiation from neural stem cells. DAPT is also utilized in cancer research, where inhibition of Notch signaling can suppress the growth of certain tumor types, especially those involving aberrant Notch activation. Additionally, it has been used in studies related to tissue regeneration, including cardiac and liver repair, due to its impact on cellular regeneration processes. Its cell-permeable nature and reversible action make it a valuable tool in in vitro and in vivo experimental models.