Used in research to selectively inhibit calpain-2, a calcium-dependent protease involved in cellular processes such as apoptosis, cytoskeletal remodeling, and signal transduction. Helps study the role of calpain-2 in neurodegenerative diseases, muscle degradation, and ischemia-reperfusion injury. Enables investigation of therapeutic strategies targeting calpain overactivation without affecting other proteases significantly. Commonly applied in cell-based assays and animal models to assess pathological mechanisms and potential neuroprotective or myoprotective effects.