Used in biochemical and cellular research to specifically inhibit calpain proteases, helping to study calpain-mediated processes such as cell signaling, apoptosis, and cytoskeletal remodeling. Frequently applied in studies related to neurodegenerative diseases, muscle degradation, and ischemia-reperfusion injury. Its high selectivity makes it valuable for controlling calpain activity in cell culture models and in vitro assays. Often used in conjunction with other protease inhibitors to maintain protein integrity in experimental systems.