Used primarily in biomedical research, it is a potent and irreversible caspase inhibitor, effectively preventing apoptosis in cell cultures. This makes it invaluable in studies focused on understanding cell death mechanisms and in experiments where preserving cell viability is crucial. It is also employed in neurobiological research to protect neurons from apoptotic pathways, aiding in the study of neurodegenerative diseases. Additionally, it is utilized in cancer research to investigate the role of apoptosis in tumor development and treatment resistance. Its ability to enhance cell survival under stress conditions further supports its use in various in vitro assays and drug discovery processes.