ERAP1-IN-1 is primarily utilized in research settings to study the role of endoplasmic reticulum aminopeptidase 1 (ERAP1) in various biological processes. It serves as a potent inhibitor of ERAP1, making it a valuable tool for investigating the enzyme's involvement in antigen processing and presentation, particularly in the context of immune responses. Researchers use this compound to explore its potential impact on autoimmune diseases, cancer immunotherapy, and infectious diseases by modulating ERAP1 activity. Additionally, it aids in understanding the molecular mechanisms underlying ERAP1's function in peptide trimming and its influence on major histocompatibility complex (MHC) class I molecules. Its application extends to drug discovery efforts aimed at developing therapeutic agents targeting ERAP1-related pathways.