This compound is primarily utilized in the field of medicinal chemistry and drug development, particularly in the synthesis of protease inhibitors. Its structure is designed to interact with specific enzyme active sites, making it valuable in the design of therapeutic agents targeting diseases such as HIV and hepatitis C. The inclusion of functional groups like the nitrobenzyl ester and acetylthio moiety allows for controlled release and targeted delivery mechanisms, enhancing the efficacy of the drug candidates. Additionally, its tert-butoxycarbonyl (Boc) protecting group ensures stability during synthetic processes, enabling precise modifications to the molecule. This compound is also explored in research for its potential in creating prodrugs, which can improve bioavailability and reduce side effects in therapeutic applications.