(1S,2S)-2-((tert-Butoxycarbonyl)amino)cyclopropanecarboxylic acid is primarily used in the field of organic synthesis and pharmaceutical research. It serves as a key intermediate in the preparation of various biologically active compounds, particularly in the synthesis of peptides and peptidomimetics. The compound is often employed in the development of protease inhibitors, which are crucial in treating diseases such as HIV and hepatitis C. Its cyclopropane ring structure contributes to the stability and conformational rigidity of the resulting molecules, enhancing their binding affinity to target proteins. Additionally, it is utilized in the construction of complex molecular architectures for drug discovery programs, where its stereochemistry plays a critical role in achieving desired pharmacological properties.