Tetranor-PGEM is primarily used as a biomarker in medical research and diagnostics to assess inflammation and prostaglandin metabolism. It is particularly valuable in studying conditions like asthma, rheumatoid arthritis, and other inflammatory diseases, as it provides insights into the activity of prostaglandin E2 (PGE2), a key mediator of inflammation. In clinical settings, measuring its levels in urine helps monitor disease progression and the effectiveness of anti-inflammatory treatments. Additionally, it is utilized in pharmacological studies to evaluate the impact of drugs targeting the prostaglandin pathway, aiding in the development of new therapeutic strategies.