Used for high-resolution separation of proteins in the molecular weight range of approximately 10–100 kDa. Ideal for analyzing complex protein mixtures where fine resolution is required, such as in proteomics research or quality control of purified proteins. The 10% acrylamide concentration provides optimal pore size for resolving proteins with small differences in molecular weight. Commonly used in Western blotting workflows to ensure clean band separation prior to transfer. Suitable for both denaturing and native PAGE, though most often used under denaturing conditions with SDS. Compatible with standard laboratory electrophoresis equipment and staining methods, including Coomassie and silver staining.