WY-14643 is widely used in research as a potent agonist of the peroxisome proliferator-activated receptor alpha (PPARα). It is primarily employed in studies focused on lipid metabolism, inflammation, and glucose homeostasis. Researchers utilize it to investigate its effects on fatty acid oxidation, making it valuable in understanding metabolic disorders like hyperlipidemia and diabetes. Additionally, it is used in experimental models to explore its potential anti-inflammatory properties and its role in regulating genes involved in energy balance. WY-14643 is also applied in toxicology studies to examine its impact on liver function and its ability to induce peroxisome proliferation. Its role in modulating PPARα pathways makes it a key tool in pharmacological and biochemical research.