Clofilium tosylate is primarily used in research settings to study cardiac arrhythmias and the mechanisms of ion channel function. It acts as a potassium channel blocker, specifically targeting the IKr (rapid delayed rectifier potassium current) in cardiac cells. This property makes it valuable for investigating the electrical activity of the heart and the effects of potassium channel inhibition on action potential duration. Researchers utilize it to model arrhythmias and to test potential antiarrhythmic drugs. Additionally, it has been employed in studies exploring the role of potassium channels in other physiological processes, such as neuronal signaling and muscle contraction. Its application is largely confined to experimental and preclinical studies, with no significant use in clinical medicine.