Wnt-C59 is primarily used in research settings to study the Wnt signaling pathway, which plays a crucial role in cell proliferation, differentiation, and tissue homeostasis. It is a potent inhibitor of Porcupine, an enzyme essential for the secretion of Wnt proteins. By blocking Porcupine, Wnt-C59 effectively suppresses Wnt signaling, making it a valuable tool for investigating the pathway's involvement in various biological processes and diseases.

In cancer research, Wnt-C59 is employed to explore its potential in inhibiting tumor growth, particularly in cancers where Wnt signaling is dysregulated, such as colorectal and breast cancer. It helps researchers understand the mechanisms of Wnt-driven oncogenesis and evaluate therapeutic strategies targeting this pathway.

Additionally, Wnt-C59 is used in stem cell studies to modulate Wnt signaling, aiding in the differentiation or maintenance of stem cells. This application is critical for regenerative medicine and tissue engineering. Its ability to finely control Wnt activity also makes it useful in developmental biology, where researchers study the pathway's role in embryogenesis and organ formation.

Overall, Wnt-C59 serves as a key experimental compound for unraveling the complexities of Wnt signaling and its implications in health and disease.