NVP-LGK974 is primarily used in research settings to study the Wnt signaling pathway, which plays a critical role in cell proliferation, differentiation, and tissue homeostasis. It is a potent inhibitor of Porcupine, an enzyme essential for the secretion of Wnt proteins. By blocking this enzyme, NVP-LGK974 helps researchers investigate the effects of Wnt signaling inhibition in various biological processes, including cancer development and stem cell regulation. Its application is particularly valuable in preclinical studies aimed at understanding the therapeutic potential of targeting Wnt signaling in diseases such as colorectal cancer, where aberrant Wnt activation is often observed. Additionally, it serves as a tool to explore the role of Wnt signaling in tissue regeneration and developmental biology.