JW74 is primarily utilized in research settings, particularly in the study of the Wnt/β-catenin signaling pathway. It functions as a potent tankyrase inhibitor, which disrupts Wnt signaling by promoting the degradation of β-catenin, making it a valuable tool for investigating the role of Wnt signaling in various biological processes, including cell proliferation, differentiation, and tissue homeostasis. Researchers employ JW74 to explore its effects on cancer biology, as aberrant Wnt/β-catenin signaling is often implicated in tumorigenesis. Additionally, it is used in developmental biology studies to understand the impact of Wnt signaling on embryogenesis and organ formation. Its application extends to drug discovery, where it serves as a reference compound for developing new therapeutics targeting Wnt-related diseases.