IWP-O1 is primarily used in biochemical research to study the Wnt signaling pathway, which plays a crucial role in cell communication, development, and tissue homeostasis. It acts as a potent inhibitor of Wnt production, effectively blocking the secretion of Wnt proteins. This makes it a valuable tool for investigating the effects of Wnt signaling inhibition in various cellular processes, such as stem cell differentiation, tissue regeneration, and cancer biology. Researchers utilize IWP-O1 to explore its potential in modulating Wnt-dependent diseases and to better understand the pathway's role in developmental and pathological conditions. Its application is particularly significant in studies aiming to develop therapeutic strategies targeting Wnt-related disorders.