Porcn Inhibitor III is primarily used in research settings to study the Wnt signaling pathway, which plays a crucial role in cell proliferation, differentiation, and tissue regeneration. By inhibiting Porcupine (Porcn), an enzyme essential for Wnt protein processing, this compound helps in understanding the mechanisms of Wnt-dependent processes. It has applications in cancer research, particularly in investigating Wnt-driven tumors, as aberrant Wnt signaling is often linked to tumor growth and metastasis. Additionally, it is used in developmental biology to explore the role of Wnt signaling in embryonic development and stem cell regulation. This inhibitor is also valuable in drug discovery efforts aimed at developing therapies for diseases associated with Wnt pathway dysregulation.