IWP-4 is widely used in biological research, particularly in the study of Wnt signaling pathways. It acts as a potent inhibitor of Wnt production, making it a valuable tool for understanding the role of Wnt proteins in various cellular processes. Researchers utilize IWP-4 to investigate its effects on stem cell differentiation, tissue regeneration, and cancer biology. By blocking Wnt signaling, it helps elucidate the mechanisms underlying developmental processes and disease progression. Additionally, IWP-4 is employed in drug discovery efforts to identify potential therapeutic targets for conditions linked to dysregulated Wnt signaling, such as certain cancers and degenerative diseases. Its application extends to in vitro studies, where it aids in creating controlled experimental conditions to observe cellular responses in the absence of Wnt activity.