GNF-6231 is primarily used in research settings as a potent and selective inhibitor of the Porcupine (PORCN) enzyme. This enzyme plays a critical role in the Wnt signaling pathway, which is involved in cell proliferation, differentiation, and tissue homeostasis. By inhibiting PORCN, GNF-6231 effectively blocks the secretion of Wnt proteins, making it a valuable tool for studying Wnt-dependent processes in various biological systems. Its application is particularly relevant in cancer research, as aberrant Wnt signaling is often associated with tumor growth and progression. Researchers utilize this compound to investigate potential therapeutic strategies for cancers driven by Wnt signaling dysregulation. Additionally, GNF-6231 is employed in developmental biology studies to explore the role of Wnt signaling in embryogenesis and tissue regeneration. Its specificity and potency make it a key compound for dissecting the complex mechanisms of Wnt-related pathways in both normal and diseased states.