Neocuproine is widely used as a chelating agent in analytical chemistry, particularly for the determination of copper ions. It forms a stable complex with copper(I), which is utilized in colorimetric assays to measure copper concentrations in various samples, including environmental and biological materials. Additionally, it serves as a catalyst in organic synthesis, especially in oxidation reactions, due to its ability to stabilize copper in its +1 oxidation state. In biochemistry, neocuproine is employed to study copper-dependent enzymes and processes, helping to elucidate the role of copper in biological systems. Its selective binding to copper(I) also makes it valuable in the development of sensors and diagnostic tools for detecting copper levels in clinical and industrial settings.