5,6-Dimethyl-1,10-phenanthroline is widely used as a chelating agent in coordination chemistry, particularly for forming stable complexes with transition metals like copper, iron, and ruthenium. These complexes are often employed in catalytic processes, including oxidation reactions and organic synthesis, due to their ability to stabilize metal ions and enhance reactivity.

In material science, it serves as a key ligand in the development of luminescent materials and sensors. Its metal complexes exhibit strong photophysical properties, making them suitable for applications in light-emitting devices and fluorescence-based detection systems.

Additionally, it is utilized in biochemical research as a probe to study metal ion interactions in biological systems, helping to elucidate mechanisms of metal-dependent enzymes and proteins. Its role in stabilizing metal centers also makes it valuable in the design of metallodrugs for therapeutic applications.