N-Acetylputrescine hydrochloride is primarily used in biochemical research as a precursor or intermediate in the synthesis of more complex organic compounds. It plays a role in studying polyamine metabolism, which is crucial for understanding cell growth, proliferation, and differentiation. This compound is also utilized in the development of pharmaceuticals, particularly in the investigation of drugs targeting polyamine-related pathways, which are implicated in various diseases, including cancer. Additionally, it serves as a reagent in laboratory experiments to explore enzymatic reactions and metabolic processes involving polyamines. Its application extends to the study of bacterial and plant physiology, where polyamines are essential for growth and stress responses.