Centrinone-B is primarily used in biological research to study cell division, particularly in the context of cancer. It functions as a potent and selective inhibitor of PLK4 (Polo-like kinase 4), a key enzyme involved in centriole duplication. By inhibiting PLK4, Centrinone-B effectively halts centriole formation, leading to centrosome depletion. This makes it a valuable tool for investigating the role of centrosomes in cell cycle regulation, genome stability, and tumorigenesis. Researchers utilize Centrinone-B to explore potential therapeutic strategies for cancers that rely on centrosome amplification for survival and proliferation. Additionally, it aids in understanding the mechanisms of centriole biogenesis and its implications in developmental biology and cellular function.