Centrinone is primarily used in biological and medical research as a potent and selective inhibitor of the enzyme PLK4 (Polo-like kinase 4). Its application is significant in studying cell division, particularly the regulation of centriole duplication, which is crucial for proper mitotic progression and genomic stability. Researchers utilize Centrinone to investigate the role of PLK4 in various cellular processes and its implications in diseases such as cancer, where centriole amplification is often observed. By inhibiting PLK4, Centrinone helps in understanding the mechanisms of centriole overduplication and its potential as a therapeutic target in cancer treatment. Additionally, it is employed in experiments aimed at developing novel anti-cancer therapies that target centrosome abnormalities.