Medicagol is primarily used in agricultural and plant science applications, particularly in the study of plant growth and development. It plays a role in enhancing root nodule formation in leguminous plants, which supports natural nitrogen fixation processes. This makes it valuable in sustainable farming practices where reducing synthetic fertilizer use is a priority. Medicagol is also investigated for its potential to improve plant resistance to environmental stress, such as drought and poor soil conditions. In research settings, it serves as a biochemical marker for studying plant-microbe interactions, especially in rhizobia-mediated symbiosis. Its natural origin and biological activity make it suitable for use in eco-friendly crop enhancement products and organic agriculture.